Articles tagged with Two Photon Microscopy
Researchers at Washington University in St. Louis have developed a new technique to visualize the coupling between neuronal activity and oxygen delivery at the cellular scale. This breakthrough could have profound implications for studying neurodegenerative disease, stroke, and other conditions connected to neurovascular coupling.
Researchers have developed a nondestructive imaging technique to measure single-cell immune metabolism in real patient blood samples, providing a higher level of information than current clinical techniques. This breakthrough could improve disease diagnosis and treatment for conditions like blood cancers, lupus, and sepsis.
Scientists at the Garvan Institute of Medical Research captured 'housekeeping' immune cells actively attacking and engulfing live melanoma cells. These macrophages patrol the edges of melanoma tumours, steadily engulfing cancer cells and slowing tumour growth. The discovery has big implications for immunotherapy.
A new study reveals that astrocytes actively participate in motor-learning circuit rewiring by eliminating synapses in the striatum. The research identifies MEGF10 as a key molecular mediator of this process, which is regulated by dopamine signaling and neural activity.
The NeuroBRIDGE project aims to transform two-photon microscopy for studying neural circuits during natural behaviors. The miniaturized, fibre-optic-based microscope enables high-resolution studies under free-moving conditions.
A new technology allows for clear observation of living retina and microglia's behavior, revealing their increased activity before tissue damage in diabetic mice. The study found that the diabetes drug liraglutide reduced microglia's activity in healthy mice too, suggesting a direct modulation mechanism.
Researchers developed a new imaging method using multiphoton microscopy to rapidly identify pancreatic neuroendocrine tumors with high accuracy. Machine learning algorithms achieved 80.6% accuracy, while convolutional neural networks outperformed with accuracies ranging from 90.8% to 96.4%.
Researchers developed a live brainstem imaging method to study the nucleus tractus solitarii's role in emotion regulation and body-brain interactions. The D-PSCAN technique enabled high-resolution visualization of NTS neural activity in response to vagus nerve stimulation and gut hormone cholecystokinin, shedding light on potential the...
Research reveals that micronuclei uptake regulates microglial morphology and gene expression, influencing neurogenesis, neural networks, and cerebrovascular function. This mechanism is crucial for the postnatal brain's development and function.
A new type of cationic epoxy photoresist exhibits greater sensitivity to two-photon laser exposure, enabling fast writing speeds and fine features. The material was developed by a research team led by Professor Cuifang Kuang, who achieved lithography speeds of 100 mm/s and resolution of 170 nm.
Researchers developed a new two-photon fluorescence microscope that captures high-speed images of neural activity at cellular resolution, providing insights into brain function and neurological diseases. The microscope uses an adaptive sampling scheme to image neurons in real time, reducing damage to brain tissue.
A study published in Neuron reveals that neurons are wired to connect seemingly unrelated concepts, enhancing the brain's ability to predict what we see based on past experiences. Visual experience influences the organisation of feedback projections, which store information about the world.
A new microscopy system called mosTF enables fast and clear imaging of the living brain, improving tracking of rapid changes in neural circuit structure. The system outperforms traditional two-photon microscopy methods by eight times speed and four-fold signal clarity.
Researchers developed a novel compound with nonlinear photochromic properties, achieving enhanced contrast and spatial resolution. The compound exhibits improved coloration efficiency with higher-intensity light, enabling diverse applications in photolithography, 3D printing, and optical disks.
A Brazilian physicist has developed an alternative method that reduces calculation time for simulating light absorption by molecules from two days to a few hours. This allows for high-resolution microscopy and the creation of precise 3D structures for data storage, with potential applications in medicinal treatments.
Researchers developed a new imaging technique using Bessel beam two-photon microscopy to detect stalling in brain capillaries, which can indicate acute neurological issues. The approach generates clear images of all capillaries every two seconds, providing better temporal resolution and enabling the detection of short stalling events.
Researchers develop low-cost 3D nanoprinting system with nanometer-level accuracy for printing microlenses, metamaterials, and micro-optical devices. The system uses a two-step absorption process and integrated fiber-coupled laser diode, making it accessible to scientists beyond optical experts.
Researchers developed an innovative imaging approach using two-photon microscopy to analyze retinal microcirculation, revealing significant changes in blood flow that may indicate brain diseases. The study suggests that microcirculation in the retina could serve as a promising predictor of cerebrovascular diseases.
Scientists have developed a new method to deliver genetic information to stem cells using nanoparticles coated with a specific polymer, enabling more efficient control over cellular differentiation. This innovation has the potential to improve the efficiency and effectiveness of regenerative medicine treatments.
A new high-speed two-photon microscope was developed with an unprecedented line scanning frequency of 400 kHz, achieving up to 10,000 frames per second. This allowed for precise observations of complex biological processes in living tissues, including calcium signal propagation and blood flow measurements.
Researchers discovered that neurons in the deepest part of the neocortex exhibit sensory responses and experience-dependent plasticity. The study found that these cells, known as L6b/surviving subplate neurons, play a role in the maturation of cortical functions.
Researchers at Shenzhen University have developed a compact fiber optical nanomechanical probe (FONP) to measure in vivo biomechanical properties of tissue and even single cells. The high-precision mechanical sensing system enables accurate measurements with spring constants as low as 2.1 nanonewtons.
Researchers mapped every thalamic synapse on 15 neurons in layer 2/3 of the visual cortex in mice and found that despite being weak and sparse, they are reliable and efficient representatives of information. The diversity of thalamic inputs underlies these advantages, allowing a small population of neurons to assemble the overall picture.
Researchers have successfully demonstrated that human brain organoids implanted in mice can establish functional connectivity and respond to external sensory stimuli, including visual cues. The breakthrough uses a combination of transparent graphene microelectrode arrays and two-photon imaging, enabling real-time observation of neural ...
A team of researchers has created a new method for fabricating nanodevices by shrinking hydrogels to create 3D patterns. This technique uses ultrafast two-photon lithography and can produce high-resolution patterns up to 13 times larger than the original size, enabling the creation of complex nanostructures.
Researchers at the University of Rochester have developed a novel 2-photon fluorescence microscopy imaging system that can detect basal cell carcinoma with perfect accuracy and squamous cell carcinoma with high accuracy. The system shortens the biopsy process to two minutes, enabling immediate treatment decisions for patients.
Researchers have developed a flexible endoscopic imaging probe using a bendable graded index (GRIN) lens, enabling 3D microscopic imaging of tissue. The new technology could shorten biopsy waiting times to minutes and enable real-time monitoring of tissue changes.
A novel 937-nm laser source has been developed for multiphoton microscopy, enabling deep tissue imaging at depths of over 600 µm with only 10 mW of power. This breakthrough technology offers a good balance between sensitivity, penetration depth, and imaging speed.
A WVU researcher is studying corollary discharge circuits in fruit flies to better understand how the brain integrates sensory information and coordinate movement. The goal of this research is to shed light on human disease and human performance, with potential applications for improving fighter pilot safety.
Customized fibers have been engineered to generate Bessel beams, opening up new applications in imaging and communications. The fibers use a technique called two-photon lithography to fabricate special beam-shaping elements, enabling the creation of compact Bessel beam generators.
Researchers will design and distribute two new types of miniscopes, enabling deeper brain observations. The devices will be light enough for rodents, unlocking insights into neurological disorders like Alzheimer's disease.
Researchers have developed a new tool to visualize leukocytes in the brain vasculature during in vivo two-photon laser scanning microscopy. The tool uses a fluorescent antibody targeting CD45, a ubiquitously expressed protein on white blood cells, allowing for tracking of circulating leukocytes over time and space.
Rice materials scientists develop a method to print arbitrary 3D shapes, creating micro-scale electronic, mechanical and photonic devices. The process involves two-photon polymerization and doping with rare earth salts for photoluminescent properties.
A team of researchers at Washington University in St. Louis has received a $3.12 million NIH grant to study neurovascular recovery after stroke. They aim to develop new neurovascular imaging technology using two-photon fluorescence microscopy and photoacoustic microscopy to visualize blood oxygen delivery in response to neuronal activity.
A novel calibration procedure developed by scientists enables precise super-resolution brain imaging at greater depth. The method corrects spherical aberration of the depletion beam, allowing for high-quality images of biological tissue.
Researchers developed a new light-sheet fluorescence microscope with extended field of view and reduced photodamage, enabling high-resolution cellular imaging over three days. The technology contributes to understanding embryonic development and pathogenesis, and is expected to advance drug development.
Kakshine is a new DNA fluorescent dye with unprecedented versatility, enabling super-resolution imaging of mitochondrial DNA in living cells and deep tissue imaging. Its applications include electrophoresis, quantitative PCR, and flow cytometry, making it a promising tool for DNA analysis.
Scientists have developed a new microscopy technique that can acquire 3D super-resolution images of subcellular structures deep inside biological tissue, including the brain. This breakthrough enables researchers to study subtle changes in neurons over time, during learning, or as a result of disease.
Researchers used holographic stimulation and calcium imaging to study the effects of acute pain on neuronal network activity. They found that spontaneous activity and synchronization between neurons increased during pain, and that blocking N-type calcium ion channels helped restore pain thresholds.
A team of researchers developed a novel microscope that can image through an intact mouse skull, resolving fine internal structures deep within living tissues. The reflection matrix microscope combines hardware and software-based adaptive optics to reconstruct object images without loss of spatial resolution.
Researchers at Max-Planck-Gesellschaft have created a miniature microscope that can capture neural activity in all cortical layers of freely behaving rats. This breakthrough technology allows for continuous imaging of neuron populations, even during complex behaviors, providing insights into the brain's circuit dynamics.
Researchers have developed a novel high-speed microscope to capture millisecond electrical signals in neurons, enabling the study of complex brain-wide interactions. The technique uses FACED technology to create a super-fast sweeping laser beam and detects voltage signals using engineered proteins.
Researchers have developed a high-speed microscope that can image the brain of an alert mouse 1,000 times a second, capturing millisecond electrical pulses through neurons. This technique allows neuroscientists to track sub-threshold inputs and identify transmission problems associated with disease.
The CUHK Faculty of Engineering has developed a novel imaging approach that enables faster 3D imaging for biomedical research. The new method uses compressive sensing and multi-focus laser scanning to reduce the number of measurements by up to 90%, resulting in significantly faster image acquisition times.
Researchers have developed a way to enhance the imaging speed of two-photon microscopy up to five times without sacrificing resolution. By combining compressive sensing with a faster scanning method, scientists can now observe biological phenomena that were previously too fleeting to image with current state-of-the-art microscopy.
The SLAP microscope uses compressed measurements to scan large areas quickly, recording neurons' voltage spikes and neurotransmitter release. It has broken the speed limit of traditional two-photon microscopy, allowing scientists to capture millisecond-scale patterns in living brains.
A novel microscopy technique, developed by Rockefeller scientists, integrates approaches to build a more cohesive picture of the brain. It captures cellular activity across large volumes of neural tissue, allowing researchers to generate a picture of rapid cellular activity across multiple layers of brain tissue.
Researchers have made novel discoveries about visual cortex layers and the subplate, a mysterious layer below. The team used optimized three-photon microscopy to measure patterns of activity among neurons in six layers of visual cortex and the subplate.
The University of Texas at San Antonio has acquired a two-photon holographic microscope to understand how incorrect wiring impacts brain function. This device will enable researchers to activate specific pathways in the brain and train neurons to respond to specific patterns of synaptic activity.
New technology tracks brain cell interactions in mice, shedding light on neuronal activity and potential insights into brain disorders such as autism and schizophrenia. The device captures three-dimensional images of neurons flashing on and off as they communicate with each other.
CU Anschutz and CU Boulder scientists have won a $2 million grant from the National Institutes of Health to refine their unique 2P-FCM microscope, which allows deeper brain imaging and dynamic focus capability. The researchers will deploy the microscope to laboratories across the country to study neural activity in various species.
Researchers have successfully visualized the development of blood vessels in zebrafish embryos without labels or contrast agents, enabling better understanding of brain and cardiovascular diseases. The new study uses optical resolution photoacoustic microscopy to provide three-dimensional images with high spatial resolution.
A special section in the Neurophotonics journal presents research in super-resolution microscopy, revealing new techniques to study neural structure and function. The findings have significant implications for understanding neurodegenerative diseases such as Alzheimer's and Parkinson's.
Researchers developed an in vivo imaging method to observe Meissner's corpuscle mechanoreceptors in living tissue using two-photon microscopy. This method could unlock the mechanism of touch sensitivity and provide a novel diagnostic tool for neural diseases. The study's findings have applications to human health, particularly in under...
Researchers at Colorado State University have developed a technique to simultaneously image with multiphoton fluorescence and second-harmonic generation, achieving nanoscale resolution. This breakthrough enables the observation of previously inaccessible structures in living tissue, opening up new avenues for biological research.
Researchers developed a new technique to selectively stiffen corneal tissue using two-photon absorption, enabling precise crosslinking without damaging the innermost layer. This approach has the potential to improve treatment outcomes for keratoconus patients and may also be useful for tissue engineering applications.
UT Southwestern Medical Center researchers have designed a powerful 3-D microscope capable of creating high-resolution images of living cancer cells in controlled microenvironments. The new approach enables detailed study of cell interactions with their environment, accelerating discovery in biology.
Researchers developed a new system to image individual neurons in the marmoset brain, overcoming limitations with two-photon microscopy. This allows for long-term study of neural activity related to cognitive and social behaviors.
The Grete Lundbeck European Brain Research Foundation has awarded TUM Prof. Arthur Konnerth the million-euro Brain Prize for his work on two-photon microscopy, enabling detailed images of individual nerve cells and synapses in living brains. His research has improved understanding of brain development, plasticity, and functional circui...
Four scientists, Winfried Denk and Arthur Konnerth (Germany), and Karel Svoboda and David Tank (USA), have been awarded the €1m Brain Prize for their invention of two-photon microscopy. This technology enables researchers to study individual nerve cells with high precision, revealing key mechanisms in brain function.